MagnetiQ Universal Pathogen DNA/RNA Extraction kit

The magnetiQ Pathogen Extraction Kit is designed to simplify the extraction of pathogenic DNA and RNA from a variety of sample types. Using buffers and magnetic beads that were optimized to capture DNA and RNA, users can obtain a purified product in 15 minutes.

Higher DNA/RNA yield from any pathogen/sample matrix

One-step magnetic bead-based extraction and purification of pathogenic DNA and RNA under 15 minutes from:

  • Swab solutions (VTM & ITM)

  • Serum/plasma

  • Blood and exudate

  • Saliva samples

  • Cell-free body fluids

  • Complex matrices such as sputum or stool

  • Cerebrospinal fluid (CSF)

The Pathogen Extraction Kit provides a safe, simple and reproducible method for obtaining purified nucleic acid products. These can be used for downstream applications such as PCR, RT-PCR and Next Generation Sequencing (NGS).

Performance

MagnetiQ Universal Pathogen DNA/RNA Kit is benchmarked against industry leading kit:

• MagMAX™ Pathogen RNA/DNA Kit (ThermoFisher) The comparison values between the ''Galenvs magnetiQ Universal Pathogen'' and "MagMAX™ Pathogen RNA/DNA Kit (ThermoFisher)'' kits were obtained following tests carried out by the Biovet Inc. laboratory.

Pathogen purification for high DNA & RNA recovery and quality of varying sizes

  • Short and scalable protocol

  • Rapid and cost-effective extractions

  • Non-toxic chemicals

  • Automatable on open liquid handling systems

Available in a range of formats designed to meet different sample size and throughput needs and designed for both manual and fully automated protocols.

Available in 50- and 200-prep manual extraction formats

Also available in prefilled plate formats for automated extraction of pathogen DNA and RNA

  • Universal Pathogen DNA / RNA Extraction Kit

    The magnetiQ Universal Pathogen Extraction kit enables researchers to obtain a pure, contaminant free product. Its chemistry is optimized to lyse cells and wash away protein, lipid, salt and any other contaminants to yield a high purity sample. Our lysis buffer disrupts the components of the matrix enabling our magnetic beads to specifically target nucleic acids, which are subsequently purified from contaminants by the washing buffers and eluted in the final step of the process.  The Pathogen Extraction kit has been optimized for 200 µl of sample from which the DNA or RNA is eluted in 50-100 µl, however there is some flexibility in the protocol. Researchers have the capacity to use a range of sample from 100-400 µl and to increase the concentration of the product by eluting in volumes as low as 50 µl. DNA and RNA can be recovered from initial concentrations as low as 1 copy/µl.

    The magnetic beads used in the lysis buffer were developed to bind to DNA as well as RNA of different sizes with high specificity, without the use of Carrier RNA. This eliminates the need for any centrifugation step, filters, requires a minimal amount of collection tubes and can be easily applied to high throughput applications. 

    It does not require any additives such as Proteinase K or RNA carrier.

  • Universal Pathogen DNA / RNA Extraction Kit

    The magnetiQ Pathogen Extraction Kit has buffers optimized for pathogenic RNA and DNA capture. The functionalized magnetic beads in the lysis buffer were developed to target and bind nucleic acids specifically. All the components are stored at room temperature.

    Sample Type:

    Swab, Saliva, Serum / Plasma, Blood, Stool, Urine, and Various transport media such as Universal Viral Transport medium (VTM), Inactive Transport Medium (ITM), Exudate, CSF

    Formats Manual: 50 or 200 assays
    Automated: 16 assays

    Elution Volume: 50-100 µL

    DNA recovered>99%

    ProcessingMode: Automated; Manual. Compatible with 96-well plates.

    Throughput: 1-250 samples per run

    Binding Technology: Magnetic beads

    Binding Capacity: Scalable

    Components:

    (i) Pathogen Lysis/Binding Buffer
    (ii) Binding Magnetic Nanoparticles
    (iii) Wash Buffer #1
    (iv) Wash Buffer #2
    (v) Elution Buffer

    Storage: Room temperature

  • Guides and protocols

    Technical Specifications

Product Codes:

  • 50 preps (UP0050)

  • 200 Preps (UP0200)

  • Prefilled plates 16-well (UP0016)

  • Prefilled plates 96-well (UP0096)

  • 100 preps (UPX0100)